Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor
Figure 1
CPA production correlates with pyocin L1 killing.
(A) Inhibition of growth of P. aeruginosa E2 and tolerant mutants M4 and M11 by pyocin L1, as shown by a soft agar overlay spot-test. 5 µl of purified pyocin L1 (1.5 mg ml−1) was spotted onto a growing lawn of cells. Clear zones indicate cell death. (B) Expression of CPA by P. aeruginosa E2 and tolerant mutants, visualised by immunoblotting with the CPA specific antibody N1F10. (C) Inhibition of growth of P. aeruginosa PAO1 and PAO1 wzm and wzt mutants by pyocin L1 (details as for A). (D) Expression of CPA by PAO1 and wzm and wzt strains (details as for B).