The Arabidopsis miR472-RDR6 Silencing Pathway Modulates PAMP- and Effector-Triggered Immunity through the Post-transcriptional Control of Disease Resistance Genes
Figure 5
MiR472 negatively regulates PTI responses and resistance against virulent Pto DC3000.
(A) Expression levels of At1g12220 and At1g51480 detected by RT-qPCR in WT, miR472OE (overexpressor) and miR472m (mutant) seedling treated with either H2O or flg22 (100 nM) for 2 hours. (B) H2O2-dependent luminescence induced by flg22 (100 nM) in WT, miR472OE and miR472m leaf discs. (C) Callose deposition induced by flg22 (100 nM) in WT, miR472OE and miR472m leaves. (D) Bacterial growth in five- to six-week-old plants from WT, miR472OE and miR472m infiltrated with Pto DC3000 (2×105 CFU mL−1). For C and D values are average ± se of four leaf discs (n = 8). Wilcoxon test was performed to determine the significant differences as compared to rdr6 plants. Asterisks “*” and “**” indicate statistically significant differences at a P value<0.05 and <0.01 respectively. These experiments were performed in two biological replicates with similar results.