Targeted Cytotoxic Therapy Kills Persisting HIV Infected Cells During ART
Figure 5
3B3-PE38 targets and systemically depletes vRNA+ cells in vivo.
Beginning on Day 28 after ART initiation 3B3-PE38 was added to the treatment regimen every other day (7 total doses: 4 at 1 µg/25 g followed by 3 at 5 µg/25 g). The ART only control includes mice treated for 28–64 days. (A) Reductions in cell-associated vRNA levels are presented as log10 differences in medians. Mann-Whitney tests were used to generate p values. (B) Reductions in cell-associated vRNA levels for all tissues in (A) are graphed alongside data from untreated mice (Wilcoxon rank-sum statistics with repeated measures corrections). (C) Quantitative ISH for No ART mice (Fig. 2), ART only mice (Fig. 2) and the ART+3B3-PE38 group revealed reductions in the total number of HIV RNA producing cells per gram. When no RNA+ cells were detected, then the number of RNA producing cells per gram tissue was set to 200 in the graph (Exact log rank tests with repeated measures corrections).