Brucella Induces an Unfolded Protein Response via TcpB That Supports Intracellular Replication in Macrophages
Figure 9
The TcpB mutant displays altered growth in macrophages.
RAW264.7 macrophages were infected with wild type (B. mel, black) or TcpB mutant Brucella (ΔTcpB, gray). Select TcpB mutant cultures were also pre-treated with tunicamycin at 0.01 µg/mL (dashed lines). At different times following infection, cells were lysed and CFU determined by transfer to dilution plates. Error bars depict standard deviations of quadruplicate determinations. *P≤0.001. Results are representative of 4 independent experiments showing altered late growth of ΔTcpB.