Infectious Prions Accumulate to High Levels in Non Proliferative C2C12 Myotubes
Figure 5
Accumulation of PrPSc in C2C12 myotubes, N2a neuroblastoma cells and SMB scrapie mouse brain cells.
(A) Time dependent increase in PrPSc in C2C12 cells. 10 µg of protein (PK−) or protein equivalent (PK+) was loaded in each lane. (B) Relative abundance of PrPSc in clinically affected mouse brain homogenate and lysates from cell lines (C2C12 myotubes, N2a neuroblastoma cells and SMB scrapie mouse brain cells). All samples are treated with PK (50 µg/mL, final concentration). Relative loading is equivalent to protein amounts prior to proteinase K digestion 0.75 µg, 7.5 µg and 75 µg respectively. Antibody SAF 83 was used.