The Effects of Somatic Hypermutation on Neutralization and Binding in the PGT121 Family of Broadly Neutralizing HIV Antibodies
Figure 9
Inferred intermediate antibodies preferentially bind native Env relative to monomeric gp120.
mAbs 3H+3L (blue) and PGT121 (red) were tested for binding by ELISA to monomeric gp120, which was extracted from lysed virus supernatants: (A) 92BR020, (C) 92RW020, (E) JR-FL E168K/N192A, (G) IAVI C22. Antibodies were also tested for cell surface Env binding by flow cytometry: (B) 92BR020, (D) 92RW020, (F) JR-FL E168K/N192A, (H) IAVI C22. mAb 2G12 was included as a control (gray). ELISA values are reported as optical density at 405 nm (OD405) and flow cytometry values are reported as mean fluorescence intensity (MFI).