The Cytotoxic Necrotizing Factor of Yersinia pseudotuberculosis (CNFY) Enhances Inflammation and Yop Delivery during Infection by Activation of Rho GTPases
Figure 5
Histopathology of H & E stained sections of the ileum and the spleen of mice orally infected with 2×108 bacteria of YPIII and YP147. (A) ileum (6 days post infection); YPIII: diffuse invasion of inflammatory cells into the lamina propria. YP147: Focal invasion of inflammatory cells into the lamina propria; adjacent tissue was unaffected. Bar represents 200 µm. White boxes indicate magnified areas in the slides below. (B) Magnified section of the ileum of an uninfected mouse (left panel), a mouse infected with YPIII - magnification of the ileum selection shown in A (middle panel), and a mouse infected with YP147- magnification of the focal invasion of inflammatory cells (right panel), local inflammation is indicated by the circle. Bar represents 50 µm. (C) spleen (6 days post infection); uninfected mouse (left panel). YPIII infected mouse: splenic atrophy and bacterial colony surrounded by focal necrosis. Pictures shown are representatives of multiple fields and samples of 9 mice. Arrow points to the bacterial foci (middle panel). YP147: hyperplasia of the white pulp and activated lymphoid follicle (right panel). W: white pulp indicated by dashed lines, N: necrosis, H: hyperplasia, R: red pulp, M: muscularis mucosa. Bar represents 50 µm.