The Cytotoxic Necrotizing Factor of Yersinia pseudotuberculosis (CNFY) Enhances Inflammation and Yop Delivery during Infection by Activation of Rho GTPases
Figure 2
Expression of PcnfY::gfp in the lymphatic tissues and organs.
2×108 bacteria of Y. pseudotuberculosis YPIII pFU228/pJNS03 (PgapA::dsred, PcnfY::gfpmut3.1) were used to orally infect BALB/c mice. Three days post infection, mice were sacrificed, and PPs, caecum, MLNs, spleen and liver were isolated. Histological slides were prepared and analyzed by fluorescence microscopy to detect bacteria in the tissues by expression of the reporter protein DsRed2. In parallel cnfY expression in the bacteria was monitored by GFPmut3.1 mediated fluorescence. White bars indicate 20 µm.