Turning Defense into Offense: Defensin Mimetics as Novel Antibiotics Targeting Lipid II
Figure 5
Mechanism of action studies of BAS00127538.
Exponentially growing S. aureus 29213 cells were exposed to compound and comparators in triplicate using 2.5% DMSO as “no drug” control. Cells were added to Mueller-Hinton Broth or M9 medium for protein synthesis and further incubated in the presence of [14C]N-acetyl glucosamine (cell wall), [3H]glycerol (lipid), [3H]Thymidine (DNA), or [3H]Leucine (protein). Following incubation, reactions were stopped by adding TCA (DNA, protein), 8% SDS (cell wall) or chloroform/methanol (lipid) and analyzed by scintillation counting.