The TgsGP Gene Is Essential for Resistance to Human Serum in Trypanosoma brucei gambiense
Figure 2
TgsGP is essential for resistance to human serum in T. b. gambiense.
The number of surviving cells after 24% human serum (open box), 10 µg/ml TLF-1 (dark grey box), 50 µg/ml recombinant APOL1 (light grey box) or a non-lytic 20% FBS control (black box). The dotted line indicates the starting concentration of 5×105 cells. The cell lines assayed were wild-type T. b. brucei; TgsGP−/+ T. b. brucei; wild-type T. b. gambiense; TgsGP−/0 T. b. gambiense; TbbHbHpR−/+ T. b. gambiense; TbbHbHpR−/+ TgsGP−/0 T. b. gambiense and TbbHbHpR−/+ TgsGP−/+ T. b. gambiense. Standard error is shown, n = 4 for each data point.