Fungal Iron Availability during Deep Seated Candidiasis Is Defined by a Complex Interplay Involving Systemic and Local Events
Figure 6
Selective chemical ablation of splenic red pulp macrophages recapitulates the renal iron accumulation phenotype in the absence of Candida infection.
Panel A. Immunohistochemical confirmation of selective ablation of splenic red pulp macrophages by clodronate [41] (100 µL of 5 mg/mL liposome-encapsulated clodronate per animal): for antibodies see Fig. 5D. Panel B. LA-ICP MS mapping of 56Fe distribution reveals medullary shift of renal iron in clodronate-treated mice. Normalised 56Fe/13C ratios across kidney following 8 h clodronate treatment are presented (see panel A); for colour scale see Fig. 2. In the bar chart (B, right) bars correspond to the percentage surface area with normalized 56Fe/13C intensity≥2-fold (left, light coloured bar) and ≥3-fold above background (right, dark coloured bar): error bars, standard deviations from the mean; n, number of biological replicates. In all cases, the experiments represent C. albicans SC5314 infections in BALB/c mice. ‘Control’, experimental control with no primary antibodies. Size bars: 200 µm. Figure in B is representative of three biological replicates.