Genome-Wide Mouse Mutagenesis Reveals CD45-Mediated T Cell Function as Critical in Protective Immunity to HSV-1
Figure 5
(A, B, C and D) The spleen of PtprcL3X/+ and PtprcL3X mice was collected and specific immune cell populations were analyzed by FACS (n = 3). Isolated splenocytes were stained for CD3, DX5 and CD19 as well as IgD and IgM; their expressions were quantified by FACS and represented as a percentage of total cells. (E) Thymocytes isolated from PtprcL3X/+ and PtprcL3X mice were stained for CD4 and CD8 and their expression were represented as a percentage of total cells. (F) Thymocytes were gated on CD4−CD8− (double negative, DN); they were then analyzed based on their CD25 and CD44 expression profiles (DN1: CD25−CD44+; DN2: CD25+CD44+; DN3: CD25+CD44−; DN4: CD25−CD44−). CD25 and CD44 expression are presented as a percentage of total CD4−CD8− double negative cells and are averaged over three animals. *, p<0.05; **, p<0.005 and ***, p<0.0005. PtprcL3X/+ and PtprcL3X are shown in white and grey, respectively (A, B, C, D, E and F).