A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies
Figure 2
Biophysical characterization of BG505 SOSIP.664 gp140 trimers.
DSC analysis of (A) purified BG505 SOSIP.664 gp140 trimers and (B) purified BG505 gp120 monomers. The melting profiles show that the trimer has a higher degree of stability than its monomeric counterpart, as its thermal transitions are initiated at a 14.4°C higher temperature. Raw data are shown in black, while the fitted curves from which Tm values were obtained are in red. (C) EM reconstruction of the BG505 SOSIP.664 gp140 trimer at 24-Å resolution. The 2D class averages and a Fourier Shell Correlation (FSC) curve are shown in Fig. S2.