Crystal Structure of Vaccinia Viral A27 Protein Reveals a Novel Structure Critical for Its Function and Complex Formation with A26 Protein
Figure 5
AUC-SV and SEC/MALS analysis of tA27 protein showing that tA27 protein formed concentration-dependent dimers.
(A) AUC analyses of tA27-WT protein. Normalized c(s) distribution plots for tA27-WT and mutants. The figure shows the distribution plots for tA27-WT at pH 7.5 (black), tA27-TM-N (green), tA27-TM-C (orange), tA27-6A (purple) and tA27-WT at pH 3.0 (red) obtained from the fitting of SV data using a continuous c(s) distribution model. (B) SEC/MALS profiles of tA27-WT protein at concentrations of 1 mg/ml (red) and 9.5 mg/ml (green). BSA was used as control (black). Thin line segments represent the calculated molar masses; the numbers denote the corresponding molecular weights of each peak (left ordinate axis) in kDa. Solid lines represent normalized UV absorbance (280 nm, right ordinate axis), and dashed lines represent light scattering.