Discovery of Anthelmintic Drug Targets and Drugs Using Chokepoints in Nematode Metabolic Pathways
Figure 5
Oxygen consumption rates in C. elegans after exposure to varying concentrations of Perhexiline (PER), Etomoxir (ETO) and Ivermectin (IVM).
A. Average basal oxygen consumption rates (OCR) of adult C. elegans incubated with vehicle (1% DMSO, 0 µM) or different concentrations of PER (CPT-2 inhibitor) (25, 50 and 100 µM) over 90 minutes. B. Average OCR of adult C. elegans incubated with vehicle (1% DMSO, Ctrl), ETO (CPT-1 inhibitor) (ETO, 50 and 100 µM), PER (50 and 100 µM), or 100 µM ETO plus 100 µM PER (ETO+PER) over 90 minutes. C. Average based OCR of adult C. elegans incubated with vehicle (1% DMSO, 0 µM) or different concentrations of IVM (binds to glutamate-gated chloride channels) over 40 minutes. D. Average OCR of adult C. elegans incubated with vehicle (1% DMSO, Ctrl), IVM (10 µM), PER (100 µM), ETO (100 µM), or 100 µM PER+100 µM ETO, over 40 minutes. Data are representative of at least 2 individual experiments. Bars represent the ±SEM of 15 OCR readings from 4 independent replicates per experiment. The experiment was repeated twice.