Trichomonas vaginalis Exosomes Deliver Cargo to Host Cells and Mediate Host∶Parasite Interactions
Figure 5
BODIPY-PC labeled exosomal membranes transfer the label to Ects.
(A) BODIPY-PC labeled exosomes or BODIPY-PC labeled hydrogenosomes were incubated with Ects. After extensive washing, cells were DAPI-stained & viewed by fluorescence microscopy. (B) Ects transiently transfected with GFPS1-10 were incubated for 1 hr with exosomes from T. vaginalis exosomes expressing GFPS11 tagged proteins. Ects were washed, fixed, DAPI-stained & viewed by fluorescent microscopy. Incubation with exosomes containing the Tvag_180840 protein tagged with the GFPS11 fragment is shown; similar data was obtained using a second (Tvag_137880) exosomal protein fused with the GFPS11 fragment. (C) Quantification of GFP fluorescence for a representative experiment, showing mean ± standard deviation. Approximately 35% of Ects were transfected using a control full length GFP (left) whereas ∼20–25% of Ects fluoresce when Ects are transfected with GFPS1-10 and incubated with either Tv_180840 exosomes (middle) or Tv_137880 exosomes (right).