Negative Regulation of Type I IFN Expression by OASL1 Permits Chronic Viral Infection and CD8+ T-Cell Exhaustion
Figure 3
Efficient induction of functional virus-specific T cells and complete viral control in Oasl1 KO mice during late phase of LCMV infection.
(A–C) Lymphocytes were isolated from the spleens of LCMV CL-13-infected WT and Oasl1 KO mice at 75 d p.i. and restimulated in vitro with GP33 or GP276 peptides for CD8+ T cells and GP66–80 (GP66) peptide for CD4+ T cells. (A) Representative data showing the percentages of cytokine-producing cells among CD8+ or CD4+ T cells. (B) Absolute numbers of T cells producing IFN-γ per spleen. (C) Summary showing the frequency of TNF-α- or IL-2-producing cells among IFN-γ+ CD8+ or IFN-γ+ CD4+ T cells. Bar graphs show mean + SD. (D) Virus titers in the kidneys extracted from LCMV CL-13-infected mice at 75 d p.i. (left) and at 130 d p.i. (right). Dashed black line represents the virus detection limit. Undetectable samples were given 20 PFU per gram. The graph includes individual values with their arithmetic mean. All data are representative of at least two independent experiments (n = 3–4 per group in each experiment). *, P<0.05; **, P<0.01; ***, P<0.001.