Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites
Figure 5
Relative expression of CPS/KS gene in rice and maize roots.
Rice and maize roots were infected with Fusarium fujikuroi spores and every 2 days RNA was isolated from three or five plants and used in real time PCR analysis. The expression levels were obtained using the delta-delta Ct and were normalized against three reference genes encoding a related actin (FFUJ_05652), a GDP-mannose transporter (FFUJ_07710) and ubiquitin (FFUJ_08398). The expression levels of CPS/KS at 4 days in maize was arbitrarily set as 1, and all other expression levels were reported relative to it.