Cryotomography of Budding Influenza A Virus Reveals Filaments with Diverse Morphologies that Mostly Do Not Bear a Genome at Their Distal End
Figure 5
Segmentation of an Archetti body.
(A) Stereo images of a segmented and isosurface rendered terminal varicosity, viewed perpendicular to the vitreous ice layer and (B) at 55° to the viewing direction in (A). Density within the bulb showed single or paired sheets (pink, green, orange, light blue, yellow) in close proximity to the membrane (grey). These features were attributed to M1. The gold fiducial markers (mustard) trace the outside edge and extent of the particle. (C) A slice through the same tomogram illustrating the presence of M1 density closely associated with the particle envelope. (D and E) Transverse sections showing that these features appear bracket shaped (black arrow) and are most likely tubes, supporting the view that these are composed of M1. See also Movie S3.