Identification of a Functional Type VI Secretion System in Campylobacter jejuni Conferring Capsule Polysaccharide Sensitive Cytotoxicity
Figure 5
Hemolysis assay depicting the T6SS-mediated hemolytic activity of C.
jejuni. (A) C. jejuni strain 108, its hcp-negative derivative 108ΔHcp, the complemented mutant strain 108ΔHcp/phcp, and the parent strain carrying phcp grown for 7-days on saponin plates and then for 16 hours in HI broth (7 p-16 h) were incubated (6 h) with red blood cells before hemolysis was determined. (B) Western blot confirming the successful restoration of Hcp secretion in 108ΔHcp after introduction of the complementation plasmid phcp. (C) Effect of the age of C. jejuni cultures in T6SS-induced hemolysis. Strain 108 and 108ΔHcp grown either on saponin plates for 3 days and then in HI broth for 8 h (3 p/8 h), or on saponin plates for 7 days and then in HI broth for 16 h (7 p/16 h) were incubated with red blood cells for 6 h. Then hemolysis was determined by measuring absorbance at 420 nm. Values are the mean ± SEM of at least three experiments.