Crosstalk between the Circadian Clock and Innate Immunity in Arabidopsis
Figure 10
Defense activation by P. syringae infection shortens the period of the ProCCA1:LUC reporter activity.
(A) Mean circadian traces for ProCCA1:LUC activity. (B) Mean circadian period of the ProCCA1:LUC reporter. Col-0 seedlings expressing the ProCCA1:LUC reporter were grown from germination in 12 hr light/12 hr dark cycles at 22°C. At ZT7, eight-day-old seedlings were incubated with PmaDG3 or PmaDG6 (1×108 or 1×107 CFU/ml, labeled as 0.1 or 0.01, respectively) for 3 mins, blot dried, and transferred to 96-well plates containing 200 µl of MS media and 30 µl of a 2.5 mM D-luciferin solution. Luciferase activity was recorded with a Packard TopCount luminometer in LL at 22°C. RAE: relative amplitude error. RAE values close to zero indicate strong rhythms while those close to 1 indicate the limit of statistically significant rhythmicity. SEM (n = 12–24) was used for (A) and (B). These experiments were repeated twice with similar results.