Crosstalk between the Circadian Clock and Innate Immunity in Arabidopsis
Figure 5
Disruption of CCA1 and LHY leads to altered stomatal activity.
(A) Stomatal aperture at ZT1 (left) or ZT13 (right) for Col-0, cca1-1, lhy-20, cca1-1lhy-20, and CCA1ox. (B) Stomatal aperture at ZT1 or ZT13 for Ler and LHYox. (C) Stomatal aperture at 1 hr (top) or 3 hr (bottom) after exposure to PmaDG3 or mock for Col-0, cca1-1, lhy-20, cca1-1lhy-20, and CCA1ox. (D) Stomatal aperture at 1 hr or 3 hr after exposure to DC3000 or mock for Ler and LHYox. For (A) and (B), three leaves from uninfected 25-day-old plants grown in 12 hr light/12 hr dark at 22°C were taken at the indicated times for the measurement of stomatal aperture. For (C) and (D), P. syringae treatment was conducted at ZT4 to ensure that most stomata were open upon treatment. Leaves were immersed in bacterial suspension (108 cfu/ml) or water as mock treatment. At least three leaves of a genotype were collected at the indicated times for stomatal aperture measurement. Data represents the average of three experiments ± SEM. Each of these experiments contains at least 70 randomly chosen stomata. Different letters in (A) indicate significant difference among the samples. Asterisks in (C) and (D) indicate significant difference between mock-treated and infected plants of the same genotype (P<0.001; Student's t-test). These experiments were repeated three times with similar results.