Crosstalk between the Circadian Clock and Innate Immunity in Arabidopsis
Figure 4
Overexpression of the LHY gene confers enhanced disease susceptibility to P. syringae.
(A) Infiltration with DC3000. (B) Spray with DC3000. 30-day-old plants were infected with P. syringae pv. tomato strain DC3000 (DC3000) by infiltration (1×105 CFU/ml) or spray (1×108 CFU/ml) at ZT1 or ZT13 in LD. Bacterial growth was assessed at 3 dpi. (C) Cell death staining. The fourth to fifth leaves of Ler and LHYox were stained with trypan blue to visualize cell death [54]. (D) SA quantification. Total SA was extracted from 20- and 30-day old plants. Data represent the average of SA levels (n = 3) ± standard deviation. Statistical analysis was performed with Student's t-test (StatView 5.0.1). Asterisks indicate significant difference between Ler and LHYox at the same time point (P<0.05). These experiments were repeated three times with similar results.