T-Cell Tropism of Simian Varicella Virus during Primary Infection
Figure 2
Macroscopic and microscopic detection of SVV-infected cells in lungs of infected African green monkeys.
(A) Macroscopic appearance of consolidated dark-red lesions (black arrow) in the lung of an SVV-wt−infected monkey at 13 dpi. (B) Macroscopic detection of EGFP fluorescence in affected area of lung (white arrow) of an SVV-EGFP-infected monkey at 9 dpi. (C) Magnification of the affected area in panel B shows EGFP fluorescence. (D–G) Serial lung sections obtained from an SVV-EGFP−infected monkey at 9 dpi analyzed by immunohistochemistry (IHC) for SVV antigens (D) or by immunofluorescence (IF) for EGFP (E), with two sections analyzed by IHC (F) or IF (G) using normal rabbit serum (NRS) and isotype control antibodies, respectively. Lung sections obtained from an SVV-wt−infected monkey at 9 dpi were analyzed using dual IF for SVV (green) and: cytokeratin (red) (H), CD3 (red) (I), CD68 (red) (J), and CD11c (red) (K) antigens. Arrows indicate double-positive cells. Asterisks indicate autofluorescent erythrocytes. Dashed lines indicate alveolar septa. Br: bronchus. Nuclei were counterstained with DAPI. D–G: 100× magnification; H, J: 400× magnification; I, K: 400× magnification and 2× digital zoom.