Guanylate-binding Protein 1 (Gbp1) Contributes to Cell-autonomous Immunity against Toxoplasma gondii
Figure 5
Cells lacking Atg5 are unable to recruit Irgb6 or Gbp1 to the parasitophorous vacuole or clear susceptible parasites.
(A) Quantification of Irgb6 localization to the parasitophorous vacuoles surrounding intracellular parasites in IFN-γ-activated BMM (50 U/ml IFN-γ, 0.1 ng/ml LPS) from Atg5flox/flox or Atg5flox/flox+LysMCre mice. Means ± S.D., n = 6 samples from 2 combined experiments. (**P<0.005, Student's t test). (B) Quantification of Gbp1 recruitment to the parasitophorous vacuoles surrounding intracellular parasites IFN-γ-activated BMM (100 U/ml IFN-γ, 0.1 ng/ml LPS) from Atg5flox/flox or Atg5flox/flox+LysMcre mice. Representative experiment from three independent experiments with similar outcomes. Mean ± S.D., n = 3 replicates per group. (*P<0.05, Student's t test). In vitro clearance of parasites in IFN-γ-activated BMM (50 U/ml IFN-γ, 0.1 ng/ml LPS) from Atg5flox/flox (C) or Atg5flox/flox+LysMcre (D) mice. Means ± S.D., n = 6 samples from 2 combined experiments. (*** P<0.001, Student's t test).