Trypanosome Infection Establishment in the Tsetse Fly Gut Is Influenced by Microbiome-Regulated Host Immune Barriers
Figure 2
Immunity-related gene expression in mature GmmWT flies following per os challenge with infectious trypanosomes.
Log2 fold-change in the expression of immunity-related genes in mature GmmWT individuals 24 hpc (A) and 3 dpc (B) with T. b. rhodesiense parasites. Gene expression in challenged and unchallenged mature GmmWT individuals is normalized relative to constitutively-expressed tsetse β-tubulin. All log2 fold-change values are represented as a fraction of average normalized gene expression levels in trypanosome-challenged vs. unchallenged flies. Samples sizes are represented by individual dots, and the red bars indicate the median log2 fold-change for each gene assayed. All quantitative measurements were performed in duplicate. Genes that presented a significant change in expression in parasite challenged versus unchallenged mature GmmWT flies are represented by red dots (p≤0.05; Student's t-test).