Differential Adaptation of Candida albicans In Vivo Modulates Immune Recognition by Dectin-1
Figure 5
Dectin-1-responsiveness but not dependence is induced by hypoxia in vitro.
(A) Growth of various strains of C. albicans under normoxia or hypoxia, as indicated. (B) Measurement of TNF responses from C57BL/6 wild type versus Clec7A−/− peritoneal macrophages after stimulation with UV-irradiated C. albicans yeast or hyphae, grown under normoxic or hypoxic conditions, as indicated. (C) Quantitation of β-glucan exposure on UV-irradiated C. albicans hyphae, grown in vitro under conditions of normoxia or hypoxia, as indicated, and stained with soluble Dectin-1. (D) Fold change in FKS gene expression in C. albicans hyphae under normoxic versus hypoxic conditions, as indicated. Data shown (mean ± SD) are from one representative experiment.