PKR-Like Endoplasmic Reticulum Kinase Is Necessary for Lipogenic Activation during HCMV Infection
Figure 5
Depletion of PERK inhibits maturation of SREBP1.
(A) and (B) Levels of SREBP2 in mock- and HCMV-infected cells. Whole cell extracts from mock- and HCMV-infected HF cells at the indicated times were analyzed to determine levels of SREBP2 precursor and mature form by Western. P, precursor SREBP2; Short, short exposure; Long, long exposure. (C) Whole cell extracts were prepared from HF cells treated with shGFP or shSREBP2 for three days and serum-starved for one day prior to mock- or HCMV-infection. Western analysis was performed by anti-SREBP2 antibody to determine the levels of the precursor and mature forms of SREBP2. M, mock infection; V, HCMV infection; P, precursor SREBP2; the arrow indicates the mature form of SREBP2. (D) The cleavage of SREBP1 and SREBP2 in PERK-depleted HF cells. HF cells were treated with shGFP or shPERK for 3 days, then mock- or HCMV-infected. Whole cell extracts were prepared at 48 hpi and analyzed by Western to determine the cleavage of SREBP1 and SREBP2. P, precursor; M, mature form; *. nonspecific protein.