Candida albicans White and Opaque Cells Undergo Distinct Programs of Filamentous Growth
Figure 5
Analysis of the role of the cAMP and MAPK signaling pathways in opaque filamentation.
MTLa strains lacking EFG1 or CPH1 transcription factors that mediate the cAMP or MAPK signaling pathway, respectively, were analyzed for their ability to undergo opaque cell filamentation. Loss of EFG1 resulted in a defect in opaque filamentation under all conditions tested (right panels), while loss of CPH1 did not have a significant effect on opaque filamentation (left panels). Strains were cultured on SOR (A), SLAD (B) or LP (C) medium for 4 days at 25°C (colonies) or 22 hours at 25°C (cells). EFG1 mutants were CAY3526 (white) and CAY3292 (opaque), CPH1 mutants were CAY3524 (white) and CAY3298 (opaque), and the EFG1 complemented strain was CAY4384 (opaque).