Vibrio parahaemolyticus Effector Proteins Suppress Inflammasome Activation by Interfering with Host Autophagy Signaling
Figure 5
The effectors VopQ and VopS are involved in the suppression of inflammasome activation.
A, B and C. BMMs from NLRP3-deficient (Nlrp3−/−) mice were primed with LPS and infected with ΔtdhAS or its respective isogenic mutants as indicated. A. Activation of caspase-1 and IL-1β processing in infected BMMs were analyzed using immunoblotting with anti-caspase-1 or anti-IL-1β antibody. B and C. IL-1β secretion into culture supernatants from infected BMMs at 3 hpi. was analyzed using an ELISA. Data are presented as the means ± SD of triplicate samples. *p<0.05.