Lipid Exchange between Borrelia burgdorferi and Host Cells
Figure 5
B. burgdorferi labeled with BODIPY-cholesterol release the fluorescent cholesterol probe in OMV.
A. BODIPY-cholesterol labeled and unlabeled B. burgdorferi release similar amounts of membrane material. DPH was added to the supernatants collected from B. burgdorferi labeled with BODIPY-cholesterol and unlabeled spirochetes to measure released OMV. Using the SpectraMaxM2, the DPH fluorescence was calculated for each sample at the specific time points. Supernatant from labeled B. burgdorferi (black); supernatant from unlabeled B. burgdorferi (gray). B. Transmission electron micrograph showing isolated vesicles from both labeled and unlabeled B. burgdorferi OMV. Immunogold labeling of OspB (18 nm) and B. burgdorferi glycolipids (6 nm) is seen throughout the vesicles. Scale Bar = 100 nM. Experiment A represents the mean ± standard error of the mean from three separate experiments.