4′-Phosphopantetheinyl Transferase PptT, a New Drug Target Required for Mycobacterium tuberculosis Growth and Persistence In Vivo
Figure 5
Effect of PptT depletion on the multiplication of M. tuberculosis H37Rv in BALB/c mice.
Mice were infected either with the wild-type M. tuberculosis strain or with strain PMM168 and either received or did not receive doxycycline treatment (0.1 mg/ml) from one day post-infection. Numbers of CFU of M. tuberculosis wild-type (dark gray bars) or of PMM168 (light gray bars) in lungs (left panel) and spleen (right panel) of treated (+) and untreated (−) mice were determined on days 1 (D1), 14 (D14) and 28 (D28) by plating dilutions of homogenized tissues on selective media. Values are means ± standard deviations (error bars) of CFU counts for three or four infected mice (see materials and methods). The dashed line corresponds to the detection limit (1.7 log). Asterisks indicate the counts in all (*) or some (**) infected mice were below the detection limit (50 CFU/organ). In such cases, the number of CFU scored was 50 CFU per organ; consequently mean number of CFU per organ is an overestimation.