TNFα-Mediated Liver Destruction by Kupffer Cells and Ly6Chi Monocytes during Entamoeba histolytica Infection
Figure 5
Role of Ly6Chi inflammatory monocytes in abscess formation.
(A) CCL2 mRNA levels in liver tissue of mice infected with E. histolytica trophozoites (ALA), sham-operated mice (sham) and naïve mice (naïve) at the indicated times (n = 4). (B) Abscess volume was determined by MRI at the indicated time points in wild-type (WT) and CCR2−/− mice. Data represent the mean ± SEM of three independent experiments (n = 3–4 animals/group). (C) Gating strategy to define liver leukocytes from wild-type (WT) and CCR2−/− mice five days post-infection. Inflammatory monocytes were defined as Ly6ChiLy6G−CD11b+ cells (region 1); Ly6Clo monocytes were defined as Ly6CloLy6G−CD11b+ cells (region 2); and neutrophils were defined as Ly6G+Ly6CloCD11b+ cells. (D) Representative MRI images of abscesses (arrows) in infected wild-type (WT) and CCR2−/− mice that received an adoptive transfer of CD115+ WT monocytes 6 hours post-infection; time post-infection is indicated. (E) MRI-based determination of abscess volume at the indicated times. Shown are representative data (mean ± SEM) of one out of two independent experiments (each 4–5 animals/group); P-values were determined by the Mann-Whitney U test (*P<0.05; **P<0.01; ***P<0.001).