Nasal Colonisation by Staphylococcus aureus Depends upon Clumping Factor B Binding to the Squamous Epithelial Cell Envelope Protein Loricrin
Figure 2
Surface Plasmon Resonance analysis of the interaction of ClfB with loricrin and keratin.
Representative sensorgrams display binding of rClfB201–542 to and dissocation from (A) GST-HLor, (B) GST-HK10, (C) GST-MLor and (D) GST-MK10 in a single cycle kinetics assay. GST-tagged ligands were captured onto a CM5 chip coated with anti-GST IgG and were exposed to increasing concentrations of rClfB201–542. Binding is measured as response units (RU) against time. The affinities were calculated from curve fitting to a plot of the RU values against concentrations of rClfB201–542. Arrows indicate the time at which rClfB201–542 is injected. The data shown is representative of 3 individual experiments.