The Calmodulin-like Calcium Binding Protein EhCaBP3 of Entamoeba histolytica Regulates Phagocytosis and Is Involved in Actin Dynamics
Figure 5
Interaction of EhCaBP3 with myosin 1B.
Total E. histolytica lysate (800 µg) was incubated with Sepharose-anti-CaBP3 antibody conjugate for 6 h at 4°C with shaking. The beads were then washed and the bound material was then eluted and analysed by western blotting and immunostained with anti-myosin 1B antibody raised in rabbit. The blot was reprobed with anti-CaBP3 antibody raised in mice. The total input lysate was also probed for the presence of EhCaBP3 and myosin 1B by their respective antibodies.