Neutrophil-derived IL-1β Is Sufficient for Abscess Formation in Immunity against Staphylococcus aureus in Mice
Figure 2
Real-time kinetics of IL-1β production and neutrophil recruitment after S. aureus skin infection.
pIL1-DsRed reporter mice or LysEGFP mice were inoculated intradermally with S. aureus. (A) Representative skin lesions (left) (entire dorsal backs [top, millimeter ruler shown for scale] and close-ups of lesions [bottom]) and mean total lesion size (cm2) ± SEM (right). (B) Representative in vivo S. aureus bioluminescence (left) and mean total flux (photons/s) ± SEM (logarithmic scale) (right). (C) Representative in vivo EGFP-neutrophil fluorescence (left) and mean total radiant efficiency (photons/s)/(µW/cm2) ± SEM (right). (D) Representative in vivo DsRed-IL-1β fluorescence (left) and mean total radiant efficiency (photons/s)/(µW/cm2) ± SEM (right). Data are from 2 experiments with at least 4 mice/group per experiment. *p<0.05; †p<0.01, S. aureus-infected mice versus none (sham injection alone) (Student's t-test).