A Heparan-Dependent Herpesvirus Targets the Olfactory Neuroepithelium for Host Entry
Figure 9
Virus uptake at the olfactory neuroepithelium.
a. 1 week old C57BL/6 mice were allowed to inhale ORF50−eGFP+ MuHV-4 (106 p.f.u. in 2 µl). The next day neuroepithelial sections were analysed for viral antigens and virus-expressed eGFP by immunofluorescent staining with polyclonal sera. Nuclei were counter-stained with DAPI. The zoomed image shows the boxed regions of the merge, with viral antigen+ neuronal cilia close to eGFP+ sustentacular cells. b. Mice were infected and analysed as in a. The zoomed images show eGFP+ sustentacular cells with viral antigen+ apical microvilli. The data are representative of >12 mice examined.