A Heparan-Dependent Herpesvirus Targets the Olfactory Neuroepithelium for Host Entry
Figure 6
Identification of neuroepithelial HS.
a. The neuroepithelia of infant and adult mice were analysed for HS expression by immunostaining with mAbs F58-10E4 (HS, sulfated) and NAH46 (HS, non-sulfated). Counter-staining was with Mayer's Hemalum. The arrows show areas of positive staining (brown). Arrowheads show the junction between neuroepithelium and respiratory epithelium. b. Tissues from naive adult mice were immunostained for HS using mAb NAH46 (brown) and counterstained with Mayer's hemalum (blue). c. Naive 1 week old mice were analysed for neuroepithelial HS expression by immunofluorescence with mAbs NAH46 and F58-10E4, and for α-tubulin to show the neuronal cilia. Nuclei were counter-stained with DAPI. The zoomed image showed the boxed region of the merge. NAH46 staining occupied the same region as the cilia, although their co-localization (yellow) was only partial.