A Hsp40 Chaperone Protein Interacts with and Modulates the Cellular Distribution of the Primase Protein of Human Cytomegalovirus
Figure 2
Schematic diagram of DNAJB6a and DNAJB6b and their deletion mutants that interact with UL70 as identified by the two hybrid screen in yeast (YTH) and co-immunoprecipitation (co-IP) in U251 cells.
DNAJB6 (a and b) proteins share the N-terminal 231 amino acids, which include the highly conserved DnaJ homology region (in blue), a Gly/Phe-rich region (in green), and a linker region (in red) but differ at the less conserved C-terminal region (in yellow and pink for DNAJB6a and b, respectively) in which a NLS (in orange) is present in the longer DNAJB6a but is absent in the shorter DNAJB6b [38], [40]. The NLS was mutated and deleted in mutants DNAJB6a-Mut and DNAJB6a-Del, respectively. The interactions that were positive and negative in the two hybrid screen or co-IP were marked as “+” and “−”, respectively.