A Tail-like Assembly at the Portal Vertex in Intact Herpes Simplex Type-1 Virions
Figure 5
Workflow for MSA-guided classification.
(A) Projected slices from a representative tomogram of HSV-1 virions. (B) 12 MSA-derived class averages, top, side and lower views. The class average representing a putative portal vertex is shown in yellow. (C) The putative portal vertex (top), the average of all other vertices (middle) and the difference map calculated from them (bottom), with the positive difference densities in blue, and negative difference density in red. (D) Final MSA-guided average, radially colored. (E) Flow chart of the procedure (described in detail within the Materials and Methods).