The Pseudomonas aeruginosa N-Acylhomoserine Lactone Quorum Sensing Molecules Target IQGAP1 and Modulate Epithelial Cell Migration
Figure 9
Visualization of 3O-C12-HSL-FITC and F-actin in Caco-2 cells.
(A) Caco-2 cell monolayers were treated with 1 µM C12-HSL-FITC (green) or 0.018% DMSO as diluent control for 1, 5, 20, or 60 min. Cells were fixed and stained with Alexa Fluor 594-conjugated phalloidin to detect F-actin (red) and DAPI nucleic acid stain (blue), and were analyzed by confocal laser scanning microscopy. The images are from one representative of at least three independent experiments. Image size is 67.6×67.6 µm and pixel size is 0.13 µm. (B) Measurement of co-localization, based on Pearson's coefficient. Columns show the mean ± standard errors (n = 10) based on three independent experiments. Significant differences (*) in mean for Pearson's coefficient compared with values for control groups as calculated by Student's t test.