The Pseudomonas aeruginosa N-Acylhomoserine Lactone Quorum Sensing Molecules Target IQGAP1 and Modulate Epithelial Cell Migration
Figure 2
3O-C12-HSL modulates migration of Caco-2 cells in a dose- and time-dependent manner.
Caco-2 cells were cultured in μ-dishes with Ibidi insert until monolayers were confluent, and the insert was removed to get two cell patches with a rectangular cell free gap (500±50 µm width). Cells were treated with 0.018% DMSO (diluent control), or 1.5, 6, 12, 25, 50, 100 and 200 µM 3O-C12-HSL and allowed to migrate. For each dish, 4 images of the migrated cells in the gap area were taken at 0, 24, 48 and 72 h. Migration rates were calculated by measuring the distance between cell monolayer patches (six measurements per image and four images per dish for the each time point) using Image J software. Shown is the mean ± standard errors of at least six independent experiments performed on separate days from different cell passages. Significant differences (* - P≤0.05; ** - P≤0.01;*** - P≤0.001) in mean for migration rate compared with values for cells in the control group as calculated by Student's t test.