Comparative Pathogenomics Reveals Horizontally Acquired Novel Virulence Genes in Fungi Infecting Cereal Hosts
Figure 5
Virulence assay of the Fusarium pseudograminearum amidohydrolase 1 mutants (ΔFpAH1) compared to the parental strain towards barley cultivar Golden Promise at 21 days post inoculation (dpi) (A–B).
(A) Survival of plants in the assay 21 dpi. N = 3 with each replicate consisting of 13–15 plants divided between two paper towel rolls and maintained in separate vessels. The difference between wild type and each mutant was highly statistically significant (P-value <0.01). (B) Representative rolls of plants from the assay. (C–D). Complementation of ΔFpAH1 (strains 96T681 and 96T682) restores virulence towards barley cultivar Golden Promise. (C) Proportion of plants surviving at 13 dpi. The total number of plants in each case was 30. (D) Photograph of assays at 13 dpi.