The Cyclase-Associated Protein Cap1 Is Important for Proper Regulation of Infection-Related Morphogenesis in Magnaporthe oryzae
Figure 5
CAP1 functions in the cAMP signaling pathway.
A. The intracellular cAMP level in Guy11, Δmac1 mutant, Ku80, Δcap1 mutant HC83, and Δcap1/CAP1 transformant CH07. Mean and standard deviation were calculated with results from three independent biological replicates. B. Appressorium formation assays with Ku80, HC83, and CH07 in the presence of 5 mM cAMP on the hydrophobic surface of GelBond membranes. C. Appressorium formation assays on the hydrophilic surface of GelBond membranes in the absence (upper panel) or presence (lower panel) of 5 mM cAMP. Bar = 10 µm.