Chemokine Receptor Ccr1 Drives Neutrophil-Mediated Kidney Immunopathology and Mortality in Invasive Candidiasis
Figure 5
Ccr1 deficiency does not affect kidney neutrophil survival or efflux of neutrophils into the urine.
(A) Representative FACS plots of annexin V and 7-AAD staining in kidney neutrophils at day 9 post-infection. Percent of (B) live annexin V− 7-AAD−, (C) apoptotic annexin V+ 7-AAD−, and (D) dead annexin V+ 7-AAD+ kidney neutrophils is similar in Ccr1+/+ and Ccr1−/− mice at days 6 and 9 post-infection. Data are shown from one of two independent experiments with similar pattern of results using a total of seven Ccr1+/+ and seven Ccr1−/− mice per time-point. (E) Accumulation of neutrophils in the urine of Ccr1+/+ and Ccr1−/− mice is similar at days 6 and 9 post-infection. Data are from two experiments with eight to ten Ccr1+/+ and eight to ten Ccr1−/− animals per time-point.