The Ebola Virus Glycoprotein Contributes to but Is Not Sufficient for Virulence In Vivo
Figure 5
Pathological evaluation of tissue samples from IFNAR−/− mice.
(A) Hematoxylin and eosin staining of tissues. Liver and spleens samples were harvested from animals (n = 3) infected with 10 ffu of either recombinant (rZEBOV and rREBOV) or chimeric (rZEBOV-RGP and rREBOV-ZGP) Ebola viruses. Samples were stained with hematoxylin and eosin prior to analysis of pathological changes. Areas of inflammatory cell infiltration are indicated with triangular arrow heads while areas of cellular necrosis are indicated with arrows. (B) Quantification of pathological changes present in tissues. Pathological changes in tissue samples were scored based on the degree of pathological change in comparison to mock infected animals (0 = normal; 1 = minimal change; 2 = mild change; 3 = moderate change; 4 = marked change; 5 = severe change). The values for each animal as well as the mean for each virus group are shown.