IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c+ Cell Populations during Visceral Leishmaniasis
Figure 5
Deletion of CD11c+ cells in DTx treated (CD11c-cre×Rosa26iDTR)F1 mice.
(A) Experimental protocol: Infected (CD11c-cre×Rosa26iDTR)F1 mice received 4 ng/g DTx i.p. at 48 hour intervals from d21 until day 28 p.i. (B and C) Impact of CD11c+ cell ablation on cells expressing CD11c and MHCII, shown by representative dot plot (B) and by extent of depletion CD11cloMHCII−, CD11cloMHCIIhi and CD11chiMHCIIhi (cDCs) relative to untreated mice (C). (D–F) Impact of DTx treatment on CD11chi cDC subsets, shown by representative dot plot (D), as total number of cells in DTx treated (black bars) vs. control (open bars) mice (E) and by extent of depletion (F). Data are from 2 pooled experiments (n = 8 mice). *** = p<0.001 for DTx vs. PBS treated groups.