The Anti-Repressor MecR2 Promotes the Proteolysis of the mecA Repressor and Enables Optimal Expression of β-lactam Resistance in MRSA
Figure 5
Effect of mecR2 on the induction of mecA transcription.
(A) Northern blot and (B) qRT-PCR analysis of the mecA induction profile in parental strain N315, mecR2 null-mutant (N315::ΔmecR2) and complemented mutant (N315::ΔmecR2+spac::mecR2, IPTG 100 µM). Cultures were induced with a sub-MIC concentration of oxacillin (0.05 mg/L) and samples were taken at 0′, 5′, 10′ 30′ and 60′.