The Anti-Repressor MecR2 Promotes the Proteolysis of the mecA Repressor and Enables Optimal Expression of β-lactam Resistance in MRSA
Figure 2
mecR2 interferes with the mecI-mediated repression of β-lactam resistance.
(A) Co-overexpression of mecI and mecR2 region (COL+mecI-mecR2) reverted the effect of mecI overexpression (COL+mecI) on the oxacillin-resistance phenotype in strain COL, as evaluated with diffusion disks containing 1 mg of oxacillin. (B) Northern blotting analysis of mecA transcription shows that in the presence of mecR2 locus the repressor effect of mecI is reverted.