Genome-Wide Identification of Pseudomonas aeruginosa Virulence-Related Genes Using a Caenorhabditis elegans Infection Model
Figure 5
ΔexoU may be a sensitized background that can reveal virulence-associated genes.
Deletion of the Type III effector protein ExoU has no statistically significant impact on PA14 virulence in C. elegans (B and D and [25]). A) Hypothetical protein PA4016 and adjacent loci PA4017 and PA4015; PA4016 is most likely a single gene transcription unit. B) A PA4016 MAR2xT7 insertion mutant (#22683) in the ΔexoU strain background has attenuated virulence in C. elegans, but a second PA4016 insertion allele (#41856) in the WT strain does not. C) Glutathione synthetase, gshB (PA0407), is a single gene transcription unit. D) Multiple alleles of gshB exhibit reduced virulence in C. elegans but the gshB #6613 allele in the ΔexoU strain background is more attenuated.